7m视频分类最新上传视频

催乳素(PRL)活性蛋白

Active Prolactin (PRL)

LTH; Luteotropic Hormone

  • 催乳素(PRL)活性蛋白 产品包装(模拟)
  • 催乳素(PRL)活性蛋白 产品包装(模拟)
  • APA846Hu61.png Figure. SDS-PAGE
  • 催乳素(PRL)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system. According to reports, PRL also stimulates proliferation of certain cells, including MCF-7. Thus, proliferation assay of PRL was conducted using MCF-7 cells. Briefly, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PRL. After incubated for 72h, cells were observed by inverted microscope and cell
proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly,10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37oC.
(A) MCF-7 cells cultured in DMEM, stimulated with 1ng/mL PRL for 72h;
(B) Unstimulated MCF-7 cells cultured in DMEM for 72h.
7m视频分类最新上传视频Figure. Cell proliferation of MCF-7 cells after stimulated with PRL.

The dose-effect curve of PRL was shown in Figure 2. It was obvious that PRL significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 3.709 to 8.973 ng/mL.
7m视频分类最新上传视频Figure. The dose-effect curve of PRL on MCF-7 cells.

用法

7m视频分类最新上传视频 Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

7m视频分类最新上传视频 避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPA846Hu61 催乳素(PRL)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA846Hu01 催乳素(PRL)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA846Hu61 催乳素(PRL)活性蛋白 Cell culture; Activity Assays.
PAA846Hu06 催乳素(PRL)多克隆抗体 WB; IHC; ICC; IP.
PAA846Hu01 催乳素(PRL)多克隆抗体 WB; IHC; ICC; IP.
LAA846Hu71 催乳素(PRL)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA846Hu22 催乳素(PRL)单克隆抗体 WB; IHC; ICC; IP.
SEA846Hu 催乳素(PRL)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA846Hu 催乳素(PRL)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA846Hu01 催乳素(PRL)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Journal of Biological Chemistry In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland
Journal of Clinical Neuroscience TLR9 expression is associated with prognosis in patients with glioblastoma multiforme
Sensors and Actuators B: Chemical Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells
Stem Cell Research & Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice
Toxicology and Applied Pharmacology The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance
Journal of Cellular Biochemistry The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway
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